| The main purpose of this project is to propose that epigenetic modifications are involved in plant tolerance under salt stress. There are three stages for the project. First, Arabidopsis homozygous mutant lines are identfied. the plant seeds were planted and genotyped during this stage. Then the salt concentration which inhibits about 50% of the wild-type Arabidopsis growth will be found and used for large-scale mutant screening. This step aims to find the salt-tolerant or sensitive mutant lines. For the last part, the gene that involves in salt stress response will be examined. The phenotypic difference between the mutant and the wild type will be verified through various methods. After the confirmation, more experiments will be done to find the possible mechanism of this gene. As freshwater is a precious resource, using seawater might be a way for future farming, and a significant proportion of cultivated land is affected by salt, detecting genes that are related to salt stress response would be helpful to promote cultivating salt-tolerant crops in the future. Previous studies on epigenetic mutations in response to salt stress have not covered all the mutated lines, this study provided more possibilities for developing salt-tolerant crops. |
