SIGNATURE WORK
CONFERENCE & EXHIBITION 2024

Liver cancer is the most frequent fatal malignancy globally. However, the widely used liver cancer treatments focused on natural compounds, chemotherapeutics, and immunotherapies, and new clinical drug production is still needed. RNA interference (RNAi) serves as a powerful tool that could temporarily knock down a gene and the most common usage is through small interfering RNA (siRNA), which requires the finding of novel targets. In this study, the Phosphodiesterase 4D Interacting Protein (PDE4DIP) and the KDEL Endoplasmic Reticulum Protein Retention Receptor 1 (KDELR1) were selected from the siRNA library by forward screening as the target genes. We wanted to explore if these genes were correlated to the cell death of liver cancer cells, especially Hep3B. To evaluate their functions, the sequences were inserted into plasmids p321 and p323 to form hairpin structures for further siRNA synthesis. The function of these genes would be tested by reverse transfection. Although the last step of transfection was not done yet, target genes were successfully inserted into the plasmids and synthesized corresponding siRNA. We could predict that PDE4DIP and KDELR1 played a significant role in liver cancer cell proliferation through previous studies. Future work should be done to complete the experiment.