Parkinson’s disease (PD) is a prevalent neurodegenerative disease that affects around 10 million people worldwide. According to past observations, early diagnosis is essential to slow down the progression of PD. The current diagnosis of PD is mainly dependent on clinical symptoms, which can be subjective and inaccurate. Other methods of diagnosis include movement data analysis from wearable electronics, transcranial sonography (TCS), blood sample analysis, and metabolomics, etc. Balancing accessibility and accuracy in diagnostic methods presents challenges for early detection, prompting the need for innovative detection strategies due to the urgency of PD diagnosis and detection. This signature work project investigates the co-aggregation between alpha-synuclein, the hall of Parkinson’s Disease (PD), and worm silk fibroin. Recombinant wild-type alpha-synuclein and V15A mutant are expressed in E.coli BL21, and co-aggregation with silk solution is examined using Raman spectroscopy. The findings of this study reveal instant interaction between the V15A alpha-synuclein and silk fibroin in vivo. This provides a foundation for a biosensing mechanism in PD detection, as the observed instant reaction is specific towards V15A, which is a pathogenic mutant of alpha-synuclein. Future directions of continued investigation on this topic may include further characterization of the biosensing selectivity, sensitivity, response time, and aggregation kinetics profile. |