OSW

SIGNATURE WORK
CONFERENCE & EXHIBITION 2023

EFFECT OF TEMPERATURE ON THE GROWTH RATE, COLONY SIZE, AND OMICS OF AN ANTARCTICA CHLORELLA STRAIN

Name

Daisy Lyu

Major

Molecular Bioscience / Genetics and Genomics

Class

2023

About

Major: Molecular Bioscience: Genetics and Genomics
Research interest: Computational Biology, Ecology and Evolutionary Biology

Signature Work Project Overview

Antarctica is considered one of the most hostile environments due to its high altitudes, extremely low temperatures, and complete darkness in winter. Incredibly, microalgae adapt themselves to this extreme environment and become the dominant producer in Antarctica. Among those inhospitable factors, temperature is critical for algae’s productivity and distribution. This study investigates the effects of temperatures on a chlorella strain collected on the coastal side of northern Ardley Island, Fildes Peninsula. Experiments were performed to measure this strain’s growth curve, colony sizes, lipidome, and metabolome at 0 °C, 4 °C, 8 °C, 12 °C, and 20 °C. Chlorella in 25 °C died in the first three weeks and thus was only measured in the colony sizes. The growth curves suggest that the optimal growing temperature of this strain ranges from 4 °C to 20 °C. Under 12 °C, linear growth was observed, while above 12 °C, a pattern between linear growth and logarithmic growth was observed. Chlorella at 0 °C and 25 °C form significantly more colonies than at other temperatures, implying colony formation might be a strategy for temperature stress. Lipid molecules and metabolites were elevated in chlorella cells at 4 °C, indicating chlorella’s response to coldness. On the other hand, chlorella at 0 °C and 25 °C was lowered in lipid molecules and metabolites compared to other temperatures, suggesting temperatures’ inhibiting effect on chlorella’s metabolic network.

Signature Work Presentation Video